John Edward Hanson

Birthdate: June 1, 1959

EDUCATION

• 1988 Ph.D. Chemistry University of California, Berkeley, CA
• 1981 B.A. Chemistry Whitman College, Walla Walla, WA

RESEARCH AND TEACHING EXPERIENCE

7/02-present

• Professor of Chemistry, University of Puget Sound
  
  Current Research Projects:
  • Synthesis of phosphonate DNA analogs as probes of the mechanism of BamH1 endonuclease.
  • Synthesis of phosphinate DNA analogs.
  • Synthesis and evaluation of substrates for T4 lysozyme.
  Teaching Load for 02/03 Academic Year
  • Chem 110 -- Fundamentals of Chemistry
  • Chem 110 Laboratory
  • Chem 250 Laboratory -- Organic Chemistry Lab
  • Chem 455 -- Computational Organic Chemistry
  • Chem 251 Laboratory -- Organic Chemistry Lab

7/97 - 7/02

• Associate Professor of Chemistry, University of Puget Sound

7/96 - 7/97

• Sabattical research at the University of Oregon: Synthesis of Substrates for T4 Lysozyme.

7/90 - 7/96

• Assistant Professor of Chemistry, University of Puget Sound

3/88 - 7/90

• Postdoctoral research at Harvard University in the lab of Don Wiley in collaboration with Jeremy Knowles, George Whitesides, and Martin Karplus. We were exploring the binding of sialic acids to influenza hemagglutinin with the eventual goal of designing tight-binding ligands that might be useful as anti-influenza agents. I was involved in synthesizing sialic acid analogs, evaluating the binding affinities of these analogs using an NMR binding assay, performing molecular mechanics calculations directed at understanding the observed binding affinities, and carrying out molecular modelling to design new ligands.

10/82 - 2/88

• Graduate research at the University of California at Berkeley under the direction of Paul A. Bartlett. By using tetrahedral phosphorus as a mimic of the transition state of peptide bond hydrolysis, we were able to produce potent inhibitors of zinc and aspartic proteases. I synthesized and tested a variety of aminoalkylphosphonic acid derivatives incorporated into appropriate peptide sequences. Because of potent inhibition (up to 1 pM), relatively slow binding (2 X 10⁵ M^-1s^-1), and, in one case, rapid decomposition (t_1/2 = 20 min), I was forced to develop unusual strategies for evaluating these inhibitors. This research formed the basis of my Ph.D. thesis: "Inhibitors of Zinc and Aspartic Peptidases: Peptides Containing Aminoalkylphosphonic Acid Derivatives".

6/80 - 5/81

• Undergraduate research at the University of Utah under the direction of Wesley Bentrude. This research was continued during my senior year at Whitman and formed the basis of my honors thesis: "The Synthesis and Thermal Decomposition of a 1,3,2-Oxazaphosphorinane and a 1,3,2-Oxazaphospholidine".

PUBLICATIONS

Hanson, J.; Hoyt, T. "Unknown Gases: Student-Designed Experiments in the Introductory Laboratory", J. Chem. Educ., 2002, 79, 845-846.

Hanson, J. "Synthesis and Use of Jacobsen's Catalyst: Enantioselective Epoxidation in the Introductory Organic Laboratory", J. Chem. Educ., 2001, 78, 1266-1268.

Fujinaga, M.; Cherney, M.M.; Tarasova, N.I.; Bartlett, P.A.; Hanson, J.E.; James, M.N.G. "Structural Study of the Complex Between Human Pepsin and a Phosphorus-Containing Peptidic Transition-State Analog", Acta Cryst., 2000, D56, 272-279.

Bartlett, P.A.; Hanson, J.E.; Morgan, B.P.; Ellsworth, B.A., "Synthesis of Peptides with a Phosphorus-Containing Amide Bond Replacement", in Houben-Weyl Methods of Organic Chemistry. In Press.

Sauter, N.K.; Hanson, J.E.; Glick, G.D.; Brown, J.H.; Crowther, R.L.; Park, S.-J.; Skehel, J.J.; Wiley, D.C., "Binding of Influenza Virus Hemagglutinin to Analogs of Its Cell-Surface Receptor, Sialic Acid: Analysis by Proton Nuclear Magnetic Resonance Spectroscopy and X-ray Crystallography", Biochemistry, 1992, 31, 9609-9621.

Hanson, J.E.; Sauter, N.K.; Skehel, J.J.; Wiley, D.C., "Proton Nuclear Magnetic Resonance Studies of the Binding of Sialosides to Intact Influenza Virus", Virology, 1992, 189, 525-533.

Fraser M.E.; Strynadka, N.C.J.; Bartlett, P.A.; Hanson, J.E.; James, M.N.G, "Crystallographic Analysis of Transition State Mimics Bound to Penicillopepsin: Phosphorus-Containing Peptide Analogues", Biochemistry, 1992, 31, 5201-5214.

Fraser, M.; Strynadka, N.; Sielecki, A.; Hanson, J.; Bartlett, P.; Gelb, M.; James, M., "Crystallographic Analyses of Peptide Inhibitors of Aspartic Proteinases", The Peptide-Protein Bridge, Toronto, Canada (Symposium Abstract), 1991.

Bartlett, P.A.; Hanson, J.E.; Giannousis, P.P., "Potent Inhibition of Pepsin and Penicillopepsin by Phosphorus-Containing Peptide Analogues", J. Org. Chem., 1990, 55, 6268-6274.

Sauter, N.K; Bednarski, M.D.; Wurzburg, B.A.; Hanson, J.E.; Whitesides, G.M.; Skehel, J.J.; Wiley, D.C., "Hemagglutinins from Two Influenza Virus Variants Bind to Sialic Acid Derivatives with Millimolar Dissociation Constants: A 500 MHz Proton Nuclear Magnetic Resonance Study", Biochemistry, 1989, 28, 8388-8396.

Hanson, J.E.; Kaplan, A.P.; Bartlett, P.A., "Phosphonate Analogs of Carboxypeptidase A Substrates are Potent Transition-State Analogue Inhibitors", Biochemistry, 1989, 28, 6294-6305.

Bartlett, P.A.; Morgan, B.P.; Drewry, D.H.; Kaplan, A.P.; Hanson, J.E.; Reich, S.H.; Shea, G.T.; Telfer, S.J.; Waterman, T.S., "Mechanism- and Structure-Derived Strategies for the Design of Biologically Active Compounds", in Proceedings of the 4th Cyprus Conference on New Methods in Drug Research, Makriyannis, A., ed., in press.

Bartlett, P.A.; Drewry, D.H.; Hanson, J.E.; Marlowe, C.K., "Details of the Interaction of Phosphorus-Containing Peptide Inhibitors with Thermolysin", in Peptides: Chemistry and Biology (Proc. Tenth Amer. Pept. Symposium), G.A. Marshall, ed. (ESCOM/Leiden, The Netherlands), 1988, pp. 427-432.

Bartlett, P.A.; Marlowe, C.K.; Giannousis, P.P.; Hanson, J.E., "Phosphorus-Containing Peptide Analogs as Peptidase Inhibitors", in Cold Spring Harbor Symposia on Quantitative Biology, Volume LII, Cold Spring Harbor Laboratory, 1987, pp. 83-90.

Bartlett, P.A.; Hanson, J.E.; Acher, F.; Giannousis, P.P., "Phosphorus Analogs as Peptidase Inhibitors: Aspartic Peptidases and Leucine Aminopeptidase", in Phosphorus Chemistry of Biomolecules, (Proc. Intl. Symp. Phos. Chem. Directed Towards Biol.), Bruzik, K.S. and Stec, W.J., eds. (Elsevier, Amsterdam), 1987, pp. 429-440.

PRESENTATIONS

AWARDS & GRANTS

PROFESSIONAL ORGANIZATIONS

American Chemical Society
Council on Undergraduate Research

RESEARCH STUDENTS

* An asterisk is included for each summer of research.